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KMID : 0861020120270060049
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Korea Journal of Herbology
2012 Volume.27 No. 6 p.49 ~ p.54
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Effects of OMC-2010 constituents on cytokine productions in mouse spleen cells
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Bae Gi-Sang
Kim Hyun-Sik
Park Kyoung-Chel
Choi Sun-Bok
Jo Il-Joo
Lee Chang-Hyuk
Seo Sang-Wan
Kim Jong-Jin
Shin Yong-Kook
Kim Min-Sun
Park Kyu-Hwan
Song Ho-Joon
Park Sung-Joo
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Abstract
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Objective: We recently reported that OMC-2010 has an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-α and interleukin (IL)-5. However, we did not find out which constituents play an important role in immuno-modulatory effect of OMC-2010. Thus, this study was performed to estimate the effects of constituents of OMC-2010 on cytokine production in mouse spleen cells, then ultimately reach to find out effective constituents regulating splenic cytokine production.
Methods: Mouse spleen cells were pre-treated with water and ethanol extract of constituents of OMC-2010 such as Rehmannia glutinosa (RG), Pinellia ternata (PT), Citrus unshiu Markovich (CUM), Glycyrrhiza uralensis (GU), Platycodon grandiflorum (PG), Schisandra chinensis (SC). After 1 h, the cells were stimulated with lipopolysaccharide (LPS, 1 ㎍/ml) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions.
Results: The water extract of RG extract significantly inhibited the LPS-induced inTNF-α and IL-5 mRNA expressions, but the water extract of PT, CUM, GU, PG, and SC did not. The ethanol extract of RG, PT, and SC significantly inhibited the LPS-induced TNF-α, and IL-5 mRNA expressions, but the ethanol extract of CUM, GU, and PG did not.
Conclusions: Theses results could suggest that the water extract of RG and the ethanol extract of RG, PT, and SC inhibited the expression of TNF-α and IL-5, which means that the possible candidate of OMC-2010 water extract’s action might be RG, and ethanol extract’s action might be RG, PR, and SC.
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KEYWORD
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OMC-2010, lipopolysaccharide (LPS), splenocyte, inflammation, Rehmannia glutinosa (RG)
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